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Novel device captures moment of tumour metastases.

A microfluidic device developed at Massachusetts General Hospital (MGH) may help study key steps in the process by which cancer cells break off from a primary tumour to invade other tissues and form metastases. The investigators have developed a stands for epithelial-mesenchymal transition, a fundamental change in cellular characteristics that has been associated with the ability of tumour cells to migrate and invade other sites in the body. Therapies that target this process may be able to slow or halt tumour metastasis.

The device is a platform to be used in testing and comparing compounds to block or delay the epithelial-mesenchymal transition, potentially slowing the progression of cancer.  Normally a stage in embryonic development, EMT is important during normal wound healing and also appears to take place when epithelial cells lining bodily surfaces and cavities become malignant. Instead of adhering to each other tightly in layers, cells that have undergone EMT gain the ability to separate out, move to other parts of the body and implant themselves into the new sites. Cells that have transitioned into a mesenchymal state appear to be more resistant to cancer therapies or other measures designed to induce cell death.

The device allows researchers to follow the movement of cells passing through a comb-like array of micropillars, which temporarily separates cells that are adhering to each other. To establish baseline characteristics of noncancerous cells, the investigators first studied the passage of normal epithelial cells through the array. They observed that those cells moved at the same speed as neighbouring cells, reconnecting when they come into contact with each other into multicellular sheets that repeatedly break apart and reseal. Tumour cells, however, passed quickly and more directly through the device and did not interact with nearby cells.

When cells in which the process of EMT had been initiated by genetic manipulation were observed passing through the device, at first they migrated collectively. But soon after encountering the first micropillars, many cells broke away from the collective front and migrated individually for the rest of their trajectory. Some cells appeared to undergo the opposite transition, reverting from individual migration back to collective migration. Subsequent analysis revealed that the slower moving cells that continued migrating together expressed epithelial markers, while the faster moving, independently migrating cells expressed mesenchymal markers. The individually cells migrating also appeared to be more resistant to treatment with chemotherapy drugs.

A particular advantage of the EMT chip is the ability to observe how the behaviour of a population of cells changes over time.  Instead of providing a snapshot of cells or tissues at a specific moment, as traditional histology studies do, the new chip can capture the changing dynamics of individual or collective cellular migration.  In the controlled environment of the EMT chip, these processes resemble such phase transitions as the change from solid to liquid that occurs with melting. Analogies with well studied physical processes are very useful for summarizing the complex EMT process into a few parameters. These parameters are very helpful when making comparisons between different cell types and studying the contribution of various biological processes to EMT. They are also useful when comparing different chemicals to discover new compounds to block or delay EMT.

Source:  Massachusetts General Hospital

 

Cells passing through EMT chip.  As cells undergoing the epithelial-mesenchymal transition move from left to right through the EMT chip, those expressing mesenchymal markers (red) break away and move independently from other cells, while cells expressing epithelial markers (green) continue to move as a collective front.  Credit: BioMEMS Resource Center, Massachusetts General Hospital.
Cells passing through EMT chip. As cells undergoing the epithelial-mesenchymal transition move from left to right through the EMT chip, those expressing mesenchymal markers (red) break away and move independently from other cells, while cells expressing epithelial markers (green) continue to move as a collective front. Credit: BioMEMS Resource Center, Massachusetts General Hospital.

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