Researchers use circulating RNA to provide multiple myeloma prognosis.


The ‘molecular mail’ sent by multiple myeloma cells provides clues to how well patients with the disease are likely to respond to treatment, according to a study being presented at the annual meeting of the American Society of Hematology (ASH) by researchers at Dana-Farber Cancer Institute.

The findings may ultimately guide doctors in deciding which therapies are best for individual patients with myeloma, the study authors say.

The study focused on exosomes, tiny sacs that cells release into the bloodstream as a way of communicating with other cells. The exosomes contain microRNA molecules, fragments of RNA that help control the activity of genes. The type of microRNA molecule in each exosome holds a specific message,  an order to be conveyed to another cell.

In the study, researchers isolated exosomes from the blood of 10 patients with myeloma and five healthy volunteers, and extracted the microRNA molecules. They found the two groups harboured sharp differences in the levels of many microRNAs.

The researchers then tested for 24 specific types of microRNAs in blood samples from 112 myeloma patients who were participating in a French clinical trial of a new drug. By tracking the results against several years of patients’ health data, they explored whether high or low levels of any of these microRNAs were associated with a particularly good or bad prognosis.

They found that patients with low amounts of two microRNAs, known as let-7e and 106b/25, survived for less time before their disease began to worsen than did the other patients.

The team state that the results indicate that blood levels of these two microRNAs can help predict progression-free survival, the average amount of time before the disease advances, in myeloma patients who have yet to be treated.  In addition to serving as a prognostic tool, the level of these microRNAs may eventually help doctors determine which patients are likely to have the best responses to different types of therapy.

Source:  Dana-Farber Cancer Institute

 

Ago HITS-CLIP decodes miRNA-mRNA interaction maps.  This research involved the generation of maps depicting gene regulation in the mouse brain by identifying miRNA-mRNA interactions in vivo, which was accomplished using the technique “Ago HITS-CLIP”. This newly-developed technique entailed purification of the Ago-miRNA-mRNA complex (which consisted of the Ago protein bound to two RNA molecules, miRNA and its target mRNA), purification of RNA using high-throughput sequencing, and computational analysis of RNAs in order to generate maps.  A 3D model of the exact mRNA molecule was obtained from the Protein Databank.  Based on a map of the mRNA indicating the miRNA binding site, a 3D graph was produced.  All 3D models were artistically laid out and the final image was rendered with Maya.  In the end, a lens flare effect was added to represent the UV irradiation and UV crosslinking site in the Ago protein.  © 2014 MultiMension All Rights Reserved.

Ago HITS-CLIP decodes miRNA-mRNA interaction maps. This research involved the generation of maps depicting gene regulation in the mouse brain by identifying miRNA-mRNA interactions in vivo, which was accomplished using the technique “Ago HITS-CLIP”. This newly-developed technique entailed purification of the Ago-miRNA-mRNA complex (which consisted of the Ago protein bound to two RNA molecules, miRNA and its target mRNA), purification of RNA using high-throughput sequencing, and computational analysis of RNAs in order to generate maps. A 3D model of the exact mRNA molecule was obtained from the Protein Databank. Based on a map of the mRNA indicating the miRNA binding site, a 3D graph was produced. All 3D models were artistically laid out and the final image was rendered with Maya. In the end, a lens flare effect was added to represent the UV irradiation and UV crosslinking site in the Ago protein. © 2014 MultiMension All Rights Reserved.

 

 

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