New technology tracks carcinogens as they are metabolised by the human body.
Researchers for the first time have developed a method to track through the human body the movement of polycyclic aromatic hydrocarbons, or PAHs, as extraordinarily tiny amounts of these potential carcinogens are biologically processed and eliminated.
PAHs, which are the product of the incomplete combustion of carbon, have been a part of everyday human life since cave dwellers first roasted meat on an open fire. More sophisticated forms of exposure now range from smoked cheese to automobile air pollution, cigarettes, a ham sandwich and public drinking water. PAHs are part of the food humans eat, the air humans breathe and the water people drink.
However, these same compounds have gained increasing interest and scientific study in recent years due to their role as carcinogens. PAHs or PAH mixtures have been named as three of the top 10 chemicals of concern by the Agency for Toxic Substances Disease Registry.
With this new technology, scientists have an opportunity to study, in a way never before possible, potential cancer-causing compounds as they move through the human body. The findings were just published by researchers from Oregon State University, Pacific Northwest National Laboratory and Lawrence Livermore National Laboratory in Chemical Research in Toxicology.
The current study has proven that this technology will work, and it’s going to change the way the medical community is able to study carcinogenic PAHs. Almost everything researchers know so far about PAH toxicity is based on giving animals high doses of the compounds and then seeing what happens. No one before this has ever been able to study these probable carcinogens at normal dietary levels and then see how they move through the body and are changed by various biological processes.
The technology allowing this to happen is a new application of accelerator mass spectrometry, which as a biological tracking tool is extraordinarily more sensitive than something like radioactivity measuring. Scientists can measure PAH levels in blood down to infinitesimal ratios, comparable to a single drop of water in 4,000 Olympic swimming pools, or to a one-inch increment on a 3-billion mile measuring tape.
As a result, microdoses of a compound, even less than one might find in a normal diet or environmental exposure, can be traced as they are processed by humans. The implications are profound. Knowing how the human body metabolize PAHs may verify a number of animal and cell studies, as well as provide a better understanding of how PAHs work, identifying their mechanism or mechanisms of action.
One PAH compound studied in this research, dibenzo (def,p)-chrysene, is fairly potent and defined as a probable human carcinogen. It was administered to volunteers in the study in a capsule equivalent to the level of PAH found in a 5-ounce serving of smoked meat, which provided about 28 percent of the average daily dietary PAH intake. There was a fairly rapid takeup of the compound, reaching a peak metabolic level within about two hours, and then rapid elimination. The researchers were able to study not only the parent compound but also individual metabolites as it was changed.
Part of what’s so interesting is that the researchers were able to administer possible carcinogens to people in scientific research and then study the results. By conventional scientific ethics, that simply would not be allowed. But from a different perspective, the scientists were not giving people toxins, they were giving them dinner. That’s how much PAHs are a part of people’s everyday lives, and now researchers are able to study these compounds at normal levels of human exposure.
What a scientist might see as a carcinogen, in other words, is what most people would see as a nice grilled steak. There are many unexpected forms of PAH exposure. The compounds are found in polluted air, cigarettes, and smoked food, of course, but also in cereal grains, potatoes and at surprisingly high levels in leafy green vegetables.
The team state that it’s clear from the research that PAHs can be toxic, but it’s also clear that there’s more to the equation than just the source of the PAH. Humans get most of the more toxic PAHs from their food, rather than inhalation. And some fairly high doses can come from foods like leafy vegetables that scientists know to be healthy. That’s why the medical community needs a better understanding of what’s going on in the human body as these compounds are processed.
The laboratory is now recruiting volunteers for a follow-up study that will also employ smoked salmon as a source of a PAH mixture and relate results to an individual’s genetic makeup.
Some of the early findings from the study actually back up previous research fairly well which was done with high-dose studies in laboratory animals. The team summise that it’s possible that exposure to dietary PAHs over a lifetime may turn out to be less of a health risk that previously believed at normal levels of exposure, but more work will need to be done with this technology before such conclusions could be reached.
Source: Oregon State University