Scientists identify new immune response via human fat cells.


When it comes to skin infections, a healthy and robust immune response may depend greatly upon what lies beneath. In a new study published in the journal Science, researchers at the University of California, report the surprising discovery that fat cells below the skin help protect humans from bacteria.  The team have uncovered a previously unknown role for dermal fat cells, known as adipocytes, they produce antimicrobial peptides that help fend off invading bacteria and other pathogens.

It was thought that once the skin barrier was broken, it was entirely the responsibility of circulating (white) blood cells like neutrophils and macrophages to protect humans from getting sepsis.  However, it takes time to recruit these cells (to the wound site). The team show in the current study that the fat stem cells are responsible for protection.  It was not known that adipocytes could produce antimicrobials, let alone that they make almost as much as a neutrophil.

The human body’s defense against microbial infection is complex, multi-tiered and involves numerous cell types, culminating in the arrival of neutrophils and monocytes, specialized cells that literally devour targeted pathogens.  But before these circulating white blood cells arrive at the scene, the body requires a more immediate response to counter the ability of many microbes to rapidly increase in number. That work is typically done by epithelial cells, mast cells and leukocytes residing in the area of infection.

Staphylococcus aureus is a common bacterium and major cause of skin and soft tissue infections in humans. The emergence of antibiotic-resistant forms of S. aureus is a significant problem worldwide in clinical medicine.

Prior studies from the lab had observed S. aureus in the fat layer of the skin, so researchers looked to see if the subcutaneous fat played a role in preventing skin infections.

The team exposed mice to S. aureus and within hours detected a major increase in both the number and size of fat cells at the site of infection. More importantly, these fat cells produced high levels of an antimicrobial peptide (AMP) called cathelicidin antimicrobial peptide or CAMP. AMPs are molecules used by the innate immune response to directly kill invasive bacteria, viruses, fungi and other pathogens.  AMPs are the natural first line defense against infection. They are evolutionarily ancient and used by all living organisms to protect themselves.

However, in humans it is becoming increasingly clear that the presence of AMPs can be a double-edged sword, particularly for CAMP. Too little CAMP and people experience frequent infections. The best example is atopic eczema (a type of recurring, itchy skin disorder). These patients can experience frequent Staph and viral infections. But too much CAMP is also bad. Evidence suggests excess CAMP can drive autoimmune and other inflammatory diseases like lupus, psoriasis and rosacea.

The scientists confirmed their findings by analyzing S. aureus infections in mice unable to either effectively produce adipocytes or whose fat cells did not express sufficient antimicrobial peptides in general and CAMP in particular. In all cases, they found the mice suffered more frequent and severe infections.  Further tests confirmed that human adipocytes also produce cathelicidin, suggesting the immune response is similar in both rodents and humans. Interestingly, obese subjects were observed to have more CAMP in their blood than subjects of normal weight.

The team state that the potential clinical applications of the findings will require further study.

Defective AMP production by mature adipocytes can occur due to obesity or insulin resistance, resulting in greater susceptibility to infection, but too much cathelicidin may provoke an unhealthy inflammatory response.

The key is that the medical community now knows this part of the immune response puzzle. It opens fantastic new options for study. For example, current drugs designed for use in diabetics might be beneficial to other people who need to boost this aspect of immunity. Conversely, these findings may help researchers understand disease associations with obesity and develop new strategies to optimize care.

Source:  Newswise, Inc

 

Activation of Zfp423 is essential for host defense against S. aureus infection. (C-D) Hematoxylin & eosin staining (C) or Perilipin (PLIN) immunostaining (D) in Zfp423+/+ and Zfp423 nur12 mouse skin after S. aureus or PBS (ctrl) injection (day 3).  Dermal adipocytes protect against invasive Staphylococcus aureus skin infection.  Gallo et al 2015.

Activation of Zfp423 is essential for host defense against S. aureus infection. (C-D) Hematoxylin & eosin staining (C) or Perilipin (PLIN) immunostaining (D) in
Zfp423+/+ and Zfp423 nur12 mouse skin after S. aureus or PBS (ctrl) injection (day 3). Dermal adipocytes protect against invasive Staphylococcus aureus skin infection. Gallo et al 2015.

 

 

 

One comment

  • ibrahim MohamedSaeed shnawa

    Adipocyte produce adipokine a type of cytokine that what is already known.The present finding is fscinating.

    Like

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