Researchers identify brain protein responsible for controlling binge- drinking.


Scientists at The Scripps Research Institute (TSRI) have discovered that a brain protein has a key role in controlling binge drinking in animal models. They found that deleting the gene for this protein in mice ramped up alcohol consumption and prevented the brain from signaling the rewarding properties of alcohol.  Alcohol hits a lot of different targets in the brain, which makes disentangling the in vivo effects of alcohol quite complicated, explain the team.  The current study sheds light on the molecular mechanisms implicated in binge drinking.  The study is published in the journal Proceedings of the National Academy of Sciences.

According to the U.S. Centers for Disease Control and Prevention, binge drinking, defined as drinking to the point of intoxication, puts people at greater risk for health problems such as cardiovascular disease, liver disease and neurological damage.

The researchers state that the goal of the current study was to identify the role of a member of the G protein-gated inwardly rectifying potassium channel (GIRK) family in the behavioural and cellular responses to alcohol.  GIRK channels are distributed throughout the nervous system, where they decrease the excitability of neurons, making them less likely to fire. Previous studies in isolated cells have revealed that alcohol can directly activate GIRK channels; however, scientists did not know whether this action matters for the behavioral effects of alcohol.

In the current study the team decided to focus on the GIRK3 subunit, which had previously been shown to modulate the effects of other drugs, such as γ-hydroxybutyrate (GHB) and cocaine.  The researchers investigated how GIRK3 influenced mouse behaviour and neuronal function in the presence of alcohol. To do so, they compared knockout mice missing GIRK3 with normal mice.

The researchers first found that the deletion of GIRK3 did not impact alcohol metabolism and did not affect how sensitive mice were to alcohol intoxication. Mice lacking GIRK3 and their normal counterparts cleared alcohol from their blood at the same rate, and experienced a similar loss of balance, sleepiness and reduced body temperature in response to alcohol.  However, in agreement with results from a previous study, the researchers found that GIRK3 knockout mice experiencing alcohol withdrawal failed to display the typical handling-induced convulsions caused by neuronal hyperexcitability.

GIRK3 knockout mice and controls also showed differences in alcohol intake in a test mirroring human behaviour during a ‘happy hour’ at a bar. In this test, mice were given access to ethanol for only two hours a day, at a time when they were most likely to drink to the point of intoxication.

The researchers found that GIRK3 knockout mice consumed much more alcohol than the control group. This effect was not observed when mice were given continuous access to alcohol, conditions under which mice do not get intoxicated. This result pointed to a role for GIRK3 specifically in binge drinking.

This led the researchers to consider two possibilities; mice lacking GIRK3 could be drinking more because they feel more pleasure from alcohol and are therefore more motivated to drink, or they could be drinking more because they feel less pleasure and therefore need to drink more to reach the same level of pleasure as normal mice.

To address this question, the researchers turned their attention to the mesocorticolimbic dopaminergic pathway, a neural circuit that facilitates reward seeking. This pathway originates in an area of the midbrain called the ventral tegmental area (VTA) and releases the neurotransmitter dopamine in two forebrain areas; the ventral striatum and the prefrontal cortex. Alcohol, like other drugs of abuse, activates this pathway.  The researchers evaluated the activity of this pathway using two complementary techniques, electrophysiological recordings, which measured the firing of VTA neurons, and microdialysis, which tracked dopamine release in the ventral striatum.

In both experiments, the baseline activity of the pathway was not affected by GIRK3 deletion. However, a striking difference between normal and GIRK3 knockout mice emerged in the presence of alcohol, as the researchers found that the pathway became completely insensitive to alcohol’s activating effect without GIRK3.  The dramatic effect of GIRK3 deletion on the ability of alcohol to excite VTA neurons was surprising to the researchers.  Even when applied at a very high concentration, alcohol was unable to alter the firing of neurons missing GIRK3.

The data findings showed that alcohol was also unable to trigger the release of dopamine in the ventral striatum of GIRK3 knockout mice.  The group hypothesize that, taken together, the results suggest that GIRK3 knockout mice drink more ethanol to boost the engagement of other neural pathways mediating alcohol’s rewarding effects.

Interestingly, the researchers were able to alter binge drinking in the opposite direction by injecting a GIRK3-expressing virus in the VTA. Reintroducing GIRK3 in the VTA of knockout mice brought their alcohol consumption down to normal levels, and normal mice expressing more GIRK3 in the VTA drank even less.

Overall, the results showed that more GIRK3 in the VTA reduced binge drinking. This has led the researchers to believe that a compound selectively targeting GIRK3-containing channels may hold promise for reducing alcohol consumption in heavy binge drinkers.

Source:  The Scripps Research Institute (TSRI) 

 

Single chromogenic GIRK3 in situ hybridization. High levels of GIRK3 expression were observed in the thalamus (Th) and cortex (Cx) of WT mice (Left), whereas expression was undetectable in the caudate-putamen (CPu) and hippocampus (Hp), as reported previously (1). Signal specificity was confirm ed by the absence of staining in KO brain sections (Right).  GIRK3 gates activation of the mesolimbic dopaminergic pathway by ethanol.   Contet et al 2015.

Single chromogenic GIRK3 in situ hybridization. High levels of GIRK3 expression were observed in the thalamus (Th) and cortex (Cx) of WT mice (Left),
whereas expression was undetectable in the caudate-putamen (CPu) and hippocampus (Hp), as reported previously (1). Signal specificity was confirm
ed by the absence of staining in KO brain sections (Right). GIRK3 gates activation of the mesolimbic dopaminergic pathway by ethanol. Contet et al 2015.

 

 

 

One comment

  • I thought it interesting that high concentrations of the gene were found in the thalamus part of the feeling brains of wild-type mice, the control group, per the above graphic. Per the study’s title, this gene presumably contributes to the overall function of the thalamus to gate information reaching the thinking brain from feeling and lower brain areas.

    And the potential causes for reduced GIRK3 expression are..? Hopefully – someday – researchers will be focused on finding causes for abnormal gene expression rather than being content to just study effects of abnormal gene expression.

    http://surfaceyourrealself.com/2015/06/07/an-inhibitory-gene-that-affects-alcohol-binge-behavior-surfaceyourrealself/

    Like

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