Skip to content

Imaging gets reinvented with new DNA microscopy technique.

Ever evolving, getting more and more minute, the imaging of cells and tissues has been a cornerstone of biology ever since cells were discovered under the light microscope centuries ago. however, advances to-date in microscopy have not incorporated the growing capability to make precise measurements of genomic sequences. Traditionally, scientists have used light, x-rays, and electrons to peer inside tissues and cells, and while microscopy illuminates spatial detail, it does not capture genetic information unless it is performed in tandem with separate genetic assays. Now, a study from researchers led by Harvard University develops a new way of mapping cell populations and visualizing how biomolecules, including different sequences of DNA and RNA, are organized spatially in cells and tissues. The team states their approach, dubbed DNA microscopy, doesn’t require any optical or other specialized equipment, instead of using synthetic nucleic acid barcodes to pinpoint molecules’ relative positions within a sample. The opensource study is published in the journal Cell.

Previous studies show while microscopy illuminates spatial detail, it does not capture genetic information unless performed in tandem with separate genetic assays. Conversely, genetic-based sequencing does not capture spatial details. What hasn’t yet been developed is a single technology possessing the capability to do the job of both optical and assay-based methods. The current study develops an imaging technique, dubbed DNA Microscopy, which spatially maps DNA and other biomolecules in cells and tissues.

The current study utilizes tiny tags, made out of customized DNA sequences, each about 30 nucleotides long, designed to latch onto every DNA and RNA molecule in a cell. Results show the tags are replicated until there are hundreds of copies of them within the cell. Data findings show when these copies interact with one another, they combine to make unique DNA labels.

The group states the interactions between these DNA tags is key, and once the labeled biomolecules are collected and sequenced, a computer algorithm decodes and reconstructs the tags’ original positions within the cell, creating a color-coded virtual image of the sample. They go on to explain pinpointing the location of each molecule is similar to how cellphone towers triangulate the locations of nearby cellphones; meaning the resulting plot is based on hundreds of thousands of different dimensions, dictated by the number of molecules with which the tagged molecules can plausibly communicate.

The team surmises they have developed a new imaging technique, namely DNA microscopy, which captures both spatial and genetic information in cells simultaneously from a single specimen. For the future, the researchers state the potential applications of DNA microscopy include looking at immune system development, nervous system architecture, and mapping genetic mutations in different cells within the same tumor in relation to their interaction with other cells and with the immune system.

Source: Howard Hughes Medical Institute (HHMI) Source


Get Healthinnovations delivered to your inbox:


Healthinnovations View All

Michelle Petersen is the founder of Healthinnovations, having worked in the health and science industry for over 21 years, which includes tenure within the NHS and Oxford University. Healthinnovations is a publication that has reported on, influenced, and researched current and future innovations in health for the past decade.

Michelle has been picked up as an expert writer for Informa publisher’s Clinical Trials community, as well as being listed as a blog source by the world’s leading medical journals, including the acclaimed Nature-Springer journal series.

Healthinnovations is currently indexed by the trusted Altmetric and PlumX metrics systems, respectively, as a blog source for published research globally. Healthinnovations is also featured in the world-renowned BioPortfolio,, the life science, pharmaceutical and healthcare portal.

Most recently the Texas A&M University covered The Top 10 Healthinnovations series on their site with distinguished Professor Stephen Maren calling the inclusion of himself and his team on the list a reflection of “the hard work and dedication of my students and trainees”.

Michelle Petersen’s copy was used in the highly successful marketing campaign for the mega-hit film ‘Jumanji: The Next Level, starring Jack Black, Karen Gilian, Kevin Hart and Dwayne ‘The Rock’ Johnson. Michelle Petersen’s copywriting was part of the film’s coverage by the Republic TV network. Republic TV is the most-watched English language TV channel in India since its inception in 2017.

An avid campaigner in the fight against child sex abuse and trafficking, Michelle is a passionate humanist striving for a better quality of life for all humans by helping to provide traction for new technologies and techniques within healthcare.

Leave a Reply

Translate »