The pathological process amyloidosis, in which misfolded proteins, amyloids, form insoluble fibril deposits, occurs in many diseases, including Alzheimer’s disease and type 2 diabetes mellitus. However, little is known about whether different forms of amyloid proteins interact or how amyloid formation begins in vivo. A new study from researchers at the Uppsala University has found evidence that amyloid from the brain can stimulate the growth of fibrils in the murine pancreas and pancreatic-related amyloid can be found along with brain-related amyloid in human brain senile plaques.
Islet amyloid can be found in islets of Langerhans in almost all patients with type 2 diabetes. Islet amyloid is made up of islet amyloid polypeptide (IAPP), which is derived from its precursor proIAPP. Accumulation of IAPP can lead to beta-cell death. In the brain, deposits of beta-amyloid in the cortex and blood vessels are characteristic findings in Alzheimer’s disease.
Several clinical studies have shown that patients with type 2 diabetes have almost a two-fold greater risk of developing Alzheimer’s disease . The data described in the current study suggest that one link between the two diseases may be the processes underlying amyloidosis.
This investigation focused on understanding how amyloid deposits seed or spread within a tissue or from one organ to another. The team explain that several soluble proteins are amyloid forming in humans. Independent of protein origin, the fibrils produced are morphologically similar. There is a potential for structures with amyloid-seeding ability to induce both homologous and heterologous fibril growth. Homologous fibril growth refers to the growth of fibrils from the same protein. Heterologous fibril growth is when fibrils from one amyloid-forming protein stimulate the growth of fibrils from a different amyloid protein. Heterologous seeding between IAPP and beta-amyloid may represent a molecular link between Alzheimer’s disease and type 2 diabetes.
In the current study the researchers first injected transgenic mice expressing human IAPP with preformed fibrils of synthetic IAPP, proIAPP, or beta-amyloid. After 10 months on a high-fat diet, tissue was analyzed using an amyloid-specific dye. The number of islets with amyloid was significantly increased compared to controls by all three types of fibrils, and the amyloid consisted of IAPP in all groups. No amyloid deposits were found in the spleen, kidney, liver, heart, or lungs. The results demonstrate for the first time that fibril injections could seed amyloid formation in the pancreas and also that brain amyloid could cross-seed fibril formation in the pancreas.
In subsequent experiments the investigators analyzed human tissues from the pancreas and brain. Using antibody-based methods, they found that pancreas sections with islet amyloid from patients diagnosed with type 2 diabetes showed no beta-amyloid immunoreactivity, whereas all samples were immunoreactive for IAPP.
To further investigate whether IAPP and beta-amyloid co-localize in human brain tissue researchers analyzed samples from the temporal cortex from Alzheimer’s disease patients and age-matched non-Alzheimer’s patients with frontotemporal dementia, progressive supranuclear palsy, or no neurological diagnosis. They found IAPP reactivity in all samples analyzed. In fact, the Alzheimer’s disease samples contained 1.4-times higher IAPP concentrations than samples from non-Alzheimer’s patients.
The team summise that it is not clear if IAPP found in brain is locally produced or derived from pancreatic beta-cells. Cross-seeding by other amyloid aggregates or perhaps by other types of aggregates offers one possible mechanism for initiation of amyloid formation. Interactions between amyloid and other aggregation-prone proteins may be of great importance in the development of protein-misfolding diseases.
Michelle Petersen is the founder of Healthinnovations, having worked in the health and science industry for over 21 years, which includes tenure within the NHS and Oxford University. Healthinnovations is a publication that has reported on, influenced, and researched current and future innovations in health for the past decade.
Michelle has been picked up as an expert writer for Informa publisher’s Clinical Trials community, as well as being listed as a blog source by the world’s leading medical journals, including the acclaimed Nature-Springer journal series.
Healthinnovations is currently indexed by the trusted Altmetric and PlumX metrics systems, respectively, as a blog source for published research globally. Healthinnovations is also featured in the world-renowned BioPortfolio, BioPortfolio.com, the life science, pharmaceutical and healthcare portal.
Most recently the Texas A&M University covered The Top 10 Healthinnovations series on their site with distinguished Professor Stephen Maren calling the inclusion of himself and his team on the list a reflection of “the hard work and dedication of my students and trainees”.
Michelle Petersen’s copy was used in the highly successful marketing campaign for the mega-hit film ‘Jumanji: The Next Level, starring Jack Black, Karen Gilian, Kevin Hart and Dwayne ‘The Rock’ Johnson. Michelle Petersen’s copywriting was part of the film’s coverage by the Republic TV network. Republic TV is the most-watched English language TV channel in India since its inception in 2017.
An avid campaigner in the fight against child sex abuse and trafficking, Michelle is a passionate humanist striving for a better quality of life for all humans by helping to provide traction for new technologies and techniques within healthcare.